Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180
SCIE
SCOPUS
Cited 21 time in
WEB OF SCIENCE
Cited 0 time in
Scopus
-
Title
- Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180
-
Author(s)
- Kim, TH; Lee, JH; Oh, YS; Bae, KS; Kim, SJ
- KIOST Author(s)
- Lee, Jung Hyun(이정현)
-
Alternative Author(s)
- 이정현; 김상진
-
Publication Year
- 1999-09
-
Abstract
- Among oil degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y lipolytica 180 degraded 94% of aliphatic hydro carbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25 degrees C. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30 degrees C, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y: lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons.
-
ISSN
- 1225-8873
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/6153
-
Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.37, no.3, pp.128 - 135, 1999
-
Publisher
- MICROBIOLOGY SOC KOREA
-
Subject
- CELL-SURFACE HYDROPHOBICITY; ADHESION MECHANISMS; HYDROCARBONS; ACINETOBACTER; DEGRADATION; ADHERENCE; STRAIN; ALKANE
-
Keywords
- Yarrowia lipolytica 180; hydrocarbon degradation; cell hydrophobicity; bioemulsification
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.