Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180 SCIE SCOPUS

Cited 20 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180
Author(s)
Kim, TH; Lee, JH; Oh, YS; Bae, KS; Kim, SJ
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
1999-09
Abstract
Among oil degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y lipolytica 180 degraded 94% of aliphatic hydro carbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25 degrees C. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30 degrees C, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y: lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/6153
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.37, no.3, pp.128 - 135, 1999
Publisher
MICROBIOLOGY SOC KOREA
Subject
CELL-SURFACE HYDROPHOBICITY; ADHESION MECHANISMS; HYDROCARBONS; ACINETOBACTER; DEGRADATION; ADHERENCE; STRAIN; ALKANE
Keywords
Yarrowia lipolytica 180; hydrocarbon degradation; cell hydrophobicity; bioemulsification
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGY SOC KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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