Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180 SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, TH | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Oh, YS | - |
dc.contributor.author | Bae, KS | - |
dc.contributor.author | Kim, SJ | - |
dc.date.accessioned | 2020-04-21T08:55:14Z | - |
dc.date.available | 2020-04-21T08:55:14Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 1999-09 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/6153 | - |
dc.description.abstract | Among oil degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y lipolytica 180 degraded 94% of aliphatic hydro carbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25 degrees C. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30 degrees C, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y: lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGY SOC KOREA | - |
dc.subject | CELL-SURFACE HYDROPHOBICITY | - |
dc.subject | ADHESION MECHANISMS | - |
dc.subject | HYDROCARBONS | - |
dc.subject | ACINETOBACTER | - |
dc.subject | DEGRADATION | - |
dc.subject | ADHERENCE | - |
dc.subject | STRAIN | - |
dc.subject | ALKANE | - |
dc.title | Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180 | - |
dc.type | Article | - |
dc.citation.endPage | 135 | - |
dc.citation.startPage | 128 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 37 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.37, no.3, pp.128 - 135 | - |
dc.identifier.wosid | 000082949700002 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CELL-SURFACE HYDROPHOBICITY | - |
dc.subject.keywordPlus | ADHESION MECHANISMS | - |
dc.subject.keywordPlus | HYDROCARBONS | - |
dc.subject.keywordPlus | ACINETOBACTER | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ADHERENCE | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | ALKANE | - |
dc.subject.keywordAuthor | Yarrowia lipolytica 180 | - |
dc.subject.keywordAuthor | hydrocarbon degradation | - |
dc.subject.keywordAuthor | cell hydrophobicity | - |
dc.subject.keywordAuthor | bioemulsification | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |