Investigation of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea SCIE SCOPUS

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Title
Investigation of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea
Author(s)
Jeong, SH; Bae, IK; Kwon, SB; Lee, JH; Jung, HI; Song, JS; Jeong, BC; Kim, SJ; Lee, SH
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2004
Abstract
Aims: Isolates obtained from various regions in Korea in 2002 were identified and their susceptibility to extended-spectrum cephalosporins, monobactams and/or cephamycins was studied along with any production of extended-spectrum beta-lactamases (ESBLs). Methods and Results: Bacteria identified by the conventional techniques and Vitek GNI card were Klebsiella pneumoniae and Escherichia coli. Using disk diffusion and double-disk synergy tests, we found that 39.2% of strains produced ESBLs. About 52% of isolates transferred resistance to ceftazidime by conjugation. Banding patterns of PCR amplification with the designed primers showed that 837- and 259-bp fragments specific to bla(TEM) genes were amplified in 63.3% of strains. 929- and 231-bp fragments (bla(SHV)), 847- and 520-bp fragments (bla(CMY)), 597- and 858-bp fragments (bla(CTX-M)) were amplified in 61.5, 17.3 and 7.7% of strains respectively. About 51.9% of strains contained more than two types of beta-lactamase genes. Especially, one strain contained bla(TEM), bla(CMY) and bla(CTX-M) genes. Significance: Resistance mechanisms to beta-lactams, comprising mostly ESBL production, lead to the resistance against even recently developed beta-lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of bla(CMY) genes and multidrug-resistant genes may also make therapeutic failure and lack of eradiation of these strains by extended-spectrum cephalosporins or cephamycins.
ISSN
0266-8254
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5365
DOI
10.1111/j.1472-765X.2004.01537.x
Bibliographic Citation
LETTERS IN APPLIED MICROBIOLOGY, v.39, no.1, pp.41 - 47, 2004
Publisher
WILEY
Subject
GRAM-NEGATIVE BACTERIA; RESISTANCE; PREVALENCE; SHV-2A
Keywords
cephamycins; CMY; CTX-M; extended-spectrum cephalosporins; SHV; TEM
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
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