Investigation of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Jeong, SH | - |
dc.contributor.author | Bae, IK | - |
dc.contributor.author | Kwon, SB | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Jung, HI | - |
dc.contributor.author | Song, JS | - |
dc.contributor.author | Jeong, BC | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Lee, SH | - |
dc.date.accessioned | 2020-04-20T15:40:31Z | - |
dc.date.available | 2020-04-20T15:40:31Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0266-8254 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/5365 | - |
dc.description.abstract | Aims: Isolates obtained from various regions in Korea in 2002 were identified and their susceptibility to extended-spectrum cephalosporins, monobactams and/or cephamycins was studied along with any production of extended-spectrum beta-lactamases (ESBLs). Methods and Results: Bacteria identified by the conventional techniques and Vitek GNI card were Klebsiella pneumoniae and Escherichia coli. Using disk diffusion and double-disk synergy tests, we found that 39.2% of strains produced ESBLs. About 52% of isolates transferred resistance to ceftazidime by conjugation. Banding patterns of PCR amplification with the designed primers showed that 837- and 259-bp fragments specific to bla(TEM) genes were amplified in 63.3% of strains. 929- and 231-bp fragments (bla(SHV)), 847- and 520-bp fragments (bla(CMY)), 597- and 858-bp fragments (bla(CTX-M)) were amplified in 61.5, 17.3 and 7.7% of strains respectively. About 51.9% of strains contained more than two types of beta-lactamase genes. Especially, one strain contained bla(TEM), bla(CMY) and bla(CTX-M) genes. Significance: Resistance mechanisms to beta-lactams, comprising mostly ESBL production, lead to the resistance against even recently developed beta-lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of bla(CMY) genes and multidrug-resistant genes may also make therapeutic failure and lack of eradiation of these strains by extended-spectrum cephalosporins or cephamycins. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.subject | GRAM-NEGATIVE BACTERIA | - |
dc.subject | RESISTANCE | - |
dc.subject | PREVALENCE | - |
dc.subject | SHV-2A | - |
dc.title | Investigation of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea | - |
dc.type | Article | - |
dc.citation.endPage | 47 | - |
dc.citation.startPage | 41 | - |
dc.citation.title | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.citation.volume | 39 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | LETTERS IN APPLIED MICROBIOLOGY, v.39, no.1, pp.41 - 47 | - |
dc.identifier.doi | 10.1111/j.1472-765X.2004.01537.x | - |
dc.identifier.wosid | 000221975400006 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GRAM-NEGATIVE BACTERIA | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | PREVALENCE | - |
dc.subject.keywordPlus | SHV-2A | - |
dc.subject.keywordAuthor | cephamycins | - |
dc.subject.keywordAuthor | CMY | - |
dc.subject.keywordAuthor | CTX-M | - |
dc.subject.keywordAuthor | extended-spectrum cephalosporins | - |
dc.subject.keywordAuthor | SHV | - |
dc.subject.keywordAuthor | TEM | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |