Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea) SCIE SCOPUS KCI

Cited 45 time in WEB OF SCIENCE Cited 54 time in Scopus
Title
Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea)
Author(s)
Song, JS; Jeon, JH; Lee, JH; Jeong, SH; Jeong, BC; Kim, SJ; Lee, JH; Lee, SH
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2005-04
Abstract
To determine the prevalence and genotypes of beta-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison scamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced beta-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum beta-lactamase. 837- and 259-bp fragments specific to bla(TEM) genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 was the most prevalent P-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type beta-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type beta-lactamases of the pre-antibiotic era indicates that TEM-type beta-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5084
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.43, no.2, pp.172 - 178, 2005
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
RESISTANCE; EVOLUTION; BACTERIA; DNA
Keywords
beta-lactamases; TEM-1; TEM-116; metagenomic library
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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