Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea)
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Title
- Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea)
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Author(s)
- Song, JS; Jeon, JH; Lee, JH; Jeong, SH; Jeong, BC; Kim, SJ; Lee, JH; Lee, SH
- KIOST Author(s)
- Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 전정호; 이정훈; 김상진; 이정현
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Publication Year
- 2005-04
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Abstract
- To determine the prevalence and genotypes of beta-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison scamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced beta-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum beta-lactamase. 837- and 259-bp fragments specific to bla(TEM) genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 was the most prevalent P-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type beta-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type beta-lactamases of the pre-antibiotic era indicates that TEM-type beta-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.
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ISSN
- 1225-8873
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/5084
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Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.43, no.2, pp.172 - 178, 2005
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Publisher
- MICROBIOLOGICAL SOCIETY KOREA
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Subject
- RESISTANCE; EVOLUTION; BACTERIA; DNA
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Keywords
- beta-lactamases; TEM-1; TEM-116; metagenomic library
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Type
- Article
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Language
- English
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Document Type
- Article
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