Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea) SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Song, JS | - |
dc.contributor.author | Jeon, JH | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Jeong, SH | - |
dc.contributor.author | Jeong, BC | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Lee, SH | - |
dc.date.accessioned | 2020-04-20T14:25:16Z | - |
dc.date.available | 2020-04-20T14:25:16Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2005-04 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/5084 | - |
dc.description.abstract | To determine the prevalence and genotypes of beta-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison scamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced beta-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum beta-lactamase. 837- and 259-bp fragments specific to bla(TEM) genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 was the most prevalent P-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type beta-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type beta-lactamases of the pre-antibiotic era indicates that TEM-type beta-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | RESISTANCE | - |
dc.subject | EVOLUTION | - |
dc.subject | BACTERIA | - |
dc.subject | DNA | - |
dc.title | Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea) | - |
dc.type | Article | - |
dc.citation.endPage | 178 | - |
dc.citation.startPage | 172 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 43 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 이정훈 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.43, no.2, pp.172 - 178 | - |
dc.identifier.scopusid | 2-s2.0-19344370271 | - |
dc.identifier.wosid | 000228956900006 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001099406 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | beta-lactamases | - |
dc.subject.keywordAuthor | TEM-1 | - |
dc.subject.keywordAuthor | TEM-116 | - |
dc.subject.keywordAuthor | metagenomic library | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |