Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase
SCIE
SCOPUS
KCI
Cited 12 time in
WEB OF SCIENCE
Cited 13 time in
Scopus
-
Title
- Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase
-
Author(s)
- Song, JS; Lee, JH; Lee, JH; Jeong, BC; Lee, WK; Lee, SH
- KIOST Author(s)
- Lee, Jung Hyun(이정현)
-
Alternative Author(s)
- 이정현
-
Publication Year
- 2006-02
-
Abstract
- This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
-
ISSN
- 1225-8873
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4924
-
Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.44, no.1, pp.126 - 128, 2006
-
Publisher
- MICROBIOLOGICAL SOCIETY KOREA
-
Subject
- MOLECULAR CHARACTERIZATION; BACTERIAL-DNA; RESISTANCE; SEQUENCES; COLI; PCR
-
Keywords
- decontamination; DNase 1; Taq DNA polymerase; TEM-type beta-lactamase
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.