Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase SCIE SCOPUS KCI
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- Title
- Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase
- KIOST Author(s)
- Lee, Jung Hyun(이정현)
- Alternative Author(s)
- 이정현
- Publication Year
- 2006-02
- Abstract
- This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
- ISSN
- 1225-8873
- Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.44, no.1, pp.126 - 128, 2006
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Subject
- MOLECULAR CHARACTERIZATION; BACTERIAL-DNA; RESISTANCE; SEQUENCES; COLI; PCR
- Keywords
- decontamination; DNase 1; Taq DNA polymerase; TEM-type beta-lactamase
- Type
- Article
- Language
- English
- Document Type
- Article
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- There are no files associated with this item.
