Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase SCIE SCOPUS KCI

Cited 11 time in WEB OF SCIENCE Cited 12 time in Scopus
Title
Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase
Author(s)
Song, JS; Lee, JH; Lee, JH; Jeong, BC; Lee, WK; Lee, SH
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2006-02
Abstract
This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4924
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.44, no.1, pp.126 - 128, 2006
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
MOLECULAR CHARACTERIZATION; BACTERIAL-DNA; RESISTANCE; SEQUENCES; COLI; PCR
Keywords
decontamination; DNase 1; Taq DNA polymerase; TEM-type beta-lactamase
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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