Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Song, JS | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Jeong, BC | - |
dc.contributor.author | Lee, WK | - |
dc.contributor.author | Lee, SH | - |
dc.date.accessioned | 2020-04-20T13:25:18Z | - |
dc.date.available | 2020-04-20T13:25:18Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4924 | - |
dc.description.abstract | This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | MOLECULAR CHARACTERIZATION | - |
dc.subject | BACTERIAL-DNA | - |
dc.subject | RESISTANCE | - |
dc.subject | SEQUENCES | - |
dc.subject | COLI | - |
dc.subject | PCR | - |
dc.title | Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase | - |
dc.type | Article | - |
dc.citation.endPage | 128 | - |
dc.citation.startPage | 126 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.44, no.1, pp.126 - 128 | - |
dc.identifier.scopusid | 2-s2.0-33646825237 | - |
dc.identifier.wosid | 000235697700018 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MOLECULAR CHARACTERIZATION | - |
dc.subject.keywordPlus | BACTERIAL-DNA | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | COLI | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordAuthor | decontamination | - |
dc.subject.keywordAuthor | DNase 1 | - |
dc.subject.keywordAuthor | Taq DNA polymerase | - |
dc.subject.keywordAuthor | TEM-type beta-lactamase | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |