A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion SCIE SCOPUS KCI

Cited 10 time in WEB OF SCIENCE Cited 10 time in Scopus
Title
A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion
Author(s)
Chung, Kyung Min; Cha, Sun-Shin; Jang, Sung Key
Publication Year
2008-09-30
Abstract
Human karyopherin beta 3, highly homologous to a yeast protein secretion enhancer (PSE1), has often been reported to be associated with a mediator of a nucleocytoplasmic transport pathway. Previously, we showed that karyopherin beta 3 complemented the PSE1 and KAP123 double mutant. Our research suggested that karyopherin beta 3 has an evolutionary function similar to that of yeast PSE1 and/or KAP 123. In this study, we performed yeast two-hybrid screening to find a protein which would interact with karyopherin beta 3 and identified apolipoprotein A-I (apo A-I), a secretion protein with a primary function in cholesterol transport. By using in vitro binding assay, coimmunoprecipitation, and colocalization studies, we defined an interaction between karyopherin beta 3 and apo A-I. In addition, overexpression of karyopherin beta 3 significantly increased apo A-I secretion. These results suggest that karyopherin beta 3 plays a crucial role in apo A-I secretion. These findings may be relevant to the study of a novel function of karyopherin beta 3 and coronary artery diseases associated with apo A-I.
ISSN
1016-8478
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4448
Bibliographic Citation
MOLECULES AND CELLS, v.26, no.3, pp.291 - 298, 2008
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Subject
NUCLEAR IMPORT RECEPTORS; MINOR CAPSID PROTEIN; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; RIBOSOMAL-PROTEINS; CHOLESTEROL EFFLUX; MAMMALIAN-CELLS; PORE COMPLEX; TRANSPORT; INTERACTS
Keywords
apolipoprotein A-I; coronary artery diseases; karyopherin beta 3; PSE1; secretion enhancer
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
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