Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 11 time in Scopus
Title
Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization
Author(s)
Sun, Younguk; Seo, Moo Seok; Kim, Jae Hyun; Kim, Yun Jae; Kim, Gun A.; Lee, Jong Il; Lee, Jung-Hyun; Kwon, Suk-Tae
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2008-12
Abstract
DNA ligases are divided into two groups according to their cofactor requirement to form ligase-adenylate, ATP-dependent DNA ligases and NAD(+)-dependent DNA ligases. The conventional view that archaeal DNA ligases only utilize ATP has recently been disputed with discoveries of dual-specificity DNA ligases (ATP/ADP or ATP/NAD(+)) from the orders Desulfurococcales and Thermococcales. Here, we studied DNA ligase encoded by the hyperthermophilic crenarchaeon Sulfophobococcus zilligii. The ligase exhibited multiple cofactor specificity utilizing ADP and GTP in addition to ATP. The unusual cofactor specificity was confirmed via a DNA ligase nick-closing activity assay using a fluorescein/biotin-labelled oligonucleotide and a radiolabelled oligonucleotide. The exploitation of GTP as a catalytic energy source has not to date been reported in any known DNA ligase. This phenomenon may provide evolutionary evidence of the nucleotide cofactor utilization by DNA ligases. To bolster this hypothesis, we summarize and evaluate previous assertions. We contend that DNA ligase evolution likely started from crenarchaeotal DNA ligases and diverged to eukaryal DNA ligases and euryarchaeotal DNA ligases. Subsequently, the NAD(+)-utilizing property of some euryarchaeotal DNA ligases may have successfully differentiated to bacterial NAD(+)-dependent DNA ligases.
ISSN
1462-2912
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4425
DOI
10.1111/j.1462-2920.2008.01710.x
Bibliographic Citation
ENVIRONMENTAL MICROBIOLOGY, v.10, no.12, pp.3212 - 3224, 2008
Publisher
WILEY
Subject
COMPLETE GENOME SEQUENCE; ARCHAEON; GENE; CLONING; ENZYME; AMPLIFICATION; EXPRESSION; RESIDUES; FIDELITY; MARINUS
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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