Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization SCIE SCOPUS

DC Field Value Language
dc.contributor.author Sun, Younguk -
dc.contributor.author Seo, Moo Seok -
dc.contributor.author Kim, Jae Hyun -
dc.contributor.author Kim, Yun Jae -
dc.contributor.author Kim, Gun A. -
dc.contributor.author Lee, Jong Il -
dc.contributor.author Lee, Jung-Hyun -
dc.contributor.author Kwon, Suk-Tae -
dc.date.accessioned 2020-04-20T10:40:13Z -
dc.date.available 2020-04-20T10:40:13Z -
dc.date.created 2020-01-28 -
dc.date.issued 2008-12 -
dc.identifier.issn 1462-2912 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4425 -
dc.description.abstract DNA ligases are divided into two groups according to their cofactor requirement to form ligase-adenylate, ATP-dependent DNA ligases and NAD(+)-dependent DNA ligases. The conventional view that archaeal DNA ligases only utilize ATP has recently been disputed with discoveries of dual-specificity DNA ligases (ATP/ADP or ATP/NAD(+)) from the orders Desulfurococcales and Thermococcales. Here, we studied DNA ligase encoded by the hyperthermophilic crenarchaeon Sulfophobococcus zilligii. The ligase exhibited multiple cofactor specificity utilizing ADP and GTP in addition to ATP. The unusual cofactor specificity was confirmed via a DNA ligase nick-closing activity assay using a fluorescein/biotin-labelled oligonucleotide and a radiolabelled oligonucleotide. The exploitation of GTP as a catalytic energy source has not to date been reported in any known DNA ligase. This phenomenon may provide evolutionary evidence of the nucleotide cofactor utilization by DNA ligases. To bolster this hypothesis, we summarize and evaluate previous assertions. We contend that DNA ligase evolution likely started from crenarchaeotal DNA ligases and diverged to eukaryal DNA ligases and euryarchaeotal DNA ligases. Subsequently, the NAD(+)-utilizing property of some euryarchaeotal DNA ligases may have successfully differentiated to bacterial NAD(+)-dependent DNA ligases. -
dc.description.uri 1 -
dc.language English -
dc.publisher WILEY -
dc.subject COMPLETE GENOME SEQUENCE -
dc.subject ARCHAEON -
dc.subject GENE -
dc.subject CLONING -
dc.subject ENZYME -
dc.subject AMPLIFICATION -
dc.subject EXPRESSION -
dc.subject RESIDUES -
dc.subject FIDELITY -
dc.subject MARINUS -
dc.title Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization -
dc.type Article -
dc.citation.endPage 3224 -
dc.citation.startPage 3212 -
dc.citation.title ENVIRONMENTAL MICROBIOLOGY -
dc.citation.volume 10 -
dc.citation.number 12 -
dc.contributor.alternativeName 김윤재 -
dc.contributor.alternativeName 이정현 -
dc.identifier.bibliographicCitation ENVIRONMENTAL MICROBIOLOGY, v.10, no.12, pp.3212 - 3224 -
dc.identifier.doi 10.1111/j.1462-2920.2008.01710.x -
dc.identifier.scopusid 2-s2.0-55849110492 -
dc.identifier.wosid 000260744800004 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus COMPLETE GENOME SEQUENCE -
dc.subject.keywordPlus ARCHAEON -
dc.subject.keywordPlus GENE -
dc.subject.keywordPlus CLONING -
dc.subject.keywordPlus ENZYME -
dc.subject.keywordPlus AMPLIFICATION -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus RESIDUES -
dc.subject.keywordPlus FIDELITY -
dc.subject.keywordPlus MARINUS -
dc.relation.journalWebOfScienceCategory Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Microbiology -
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