Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli SCIE SCOPUS

Cited 42 time in WEB OF SCIENCE Cited 51 time in Scopus
Title
Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli
Author(s)
Tam Dinh Le Vo; Kim, Tae Wan; Hong, Soon Ho
Publication Year
2012-05
Abstract
Gamma-aminobutyric acid (GABA) is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of l-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.46 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.5%.
ISSN
1615-7591
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3590
DOI
10.1007/s00449-011-0634-8
Bibliographic Citation
BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, no.4, pp.645 - 650, 2012
Publisher
SPRINGER
Subject
BROWN RICE EXTRACTS; LACTOBACILLUS-BREVIS; ENHANCED LEVELS; RESISTANCE; EXPRESSION
Keywords
gamma-aminobutyric acid; Bioconversion; Glutamate decarboxylase; Glutamate/GABA antiporter; GABA aminotransferase
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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