Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli
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Title
- Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli
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Author(s)
- Tam Dinh Le Vo; Kim, Tae Wan; Hong, Soon Ho
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Alternative Author(s)
- 김태완
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Publication Year
- 2012-05
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Abstract
- Gamma-aminobutyric acid (GABA) is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of l-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.46 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.5%.
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ISSN
- 1615-7591
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3590
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DOI
- 10.1007/s00449-011-0634-8
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Bibliographic Citation
- BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, no.4, pp.645 - 650, 2012
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Publisher
- SPRINGER
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Subject
- BROWN RICE EXTRACTS; LACTOBACILLUS-BREVIS; ENHANCED LEVELS; RESISTANCE; EXPRESSION
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Keywords
- gamma-aminobutyric acid; Bioconversion; Glutamate decarboxylase; Glutamate/GABA antiporter; GABA aminotransferase
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Type
- Article
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Language
- English
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Document Type
- Article
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