Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tam Dinh Le Vo | - |
dc.contributor.author | Kim, Tae Wan | - |
dc.contributor.author | Hong, Soon Ho | - |
dc.date.accessioned | 2020-04-20T06:55:17Z | - |
dc.date.available | 2020-04-20T06:55:17Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3590 | - |
dc.description.abstract | Gamma-aminobutyric acid (GABA) is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of l-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.46 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.5%. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | BROWN RICE EXTRACTS | - |
dc.subject | LACTOBACILLUS-BREVIS | - |
dc.subject | ENHANCED LEVELS | - |
dc.subject | RESISTANCE | - |
dc.subject | EXPRESSION | - |
dc.title | Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli | - |
dc.type | Article | - |
dc.citation.endPage | 650 | - |
dc.citation.startPage | 645 | - |
dc.citation.title | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.citation.volume | 35 | - |
dc.citation.number | 4 | - |
dc.contributor.alternativeName | 김태완 | - |
dc.identifier.bibliographicCitation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, no.4, pp.645 - 650 | - |
dc.identifier.doi | 10.1007/s00449-011-0634-8 | - |
dc.identifier.scopusid | 2-s2.0-84861482911 | - |
dc.identifier.wosid | 000303053900016 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LACTOBACILLUS-BREVIS | - |
dc.subject.keywordPlus | ENHANCED LEVELS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BROWN RICE EXTRACTS | - |
dc.subject.keywordAuthor | gamma-aminobutyric acid | - |
dc.subject.keywordAuthor | Bioconversion | - |
dc.subject.keywordAuthor | Glutamate decarboxylase | - |
dc.subject.keywordAuthor | Glutamate/GABA antiporter | - |
dc.subject.keywordAuthor | GABA aminotransferase | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |