해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성
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Title
- 해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성
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Alternative Title
- Enantioselective Production of Levofloxacin by a Microbial Lipase : Screening and Characterization of the Lipase from the Yeast Yarrowia lipolytica 180
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Author(s)
- 김준태; 서해점; 우정희; 강성균; 정병철; 이정현; 김상진
- KIOST Author(s)
- Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 김준태; 우정희; 강성균; 이정현; 김상진
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Publication Year
- 2005-05-12
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Abstract
- Levofloxacin, (S)-isomer of the (R),(S)-ofloxacin, is a new fluoroquinolone antibiotic. To screen strains producing a lipase that hydrolyzes the (R),(S)-ofloxacin ester to levofloxacin, 151 isolates showing lipolytic activity on tributyrin agar plate were tested for the hydrolysis of ofloxacin ester. 3 strains among them were selected, and one of them, identified as the yeast Yarrowia lipolytica CL180, was found to enantioselectively hydrolyze the (S)-enantiomer of the (R),(S)-ofloxacin ester. The enantioselective lipase-encoding gene was cloned by screening genomic library of Y. lipolytica CL 180 and the sequence was determined. The lipase gene consisted of 1431-bp nucleotides, encoding a polypeptide of 476 amino acids with a molecular mass of 52 kDa. The consensus sequence G-X1-S-X2-G in most serine lipases was well conserved. The catalytic apparatus involving the triad serine, aspartate, and histidine was also found in the protein sequence. The amino acids comparison showed a high similarity with the yeast lipases of type B1 carboxylesterase/lipase family.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/31482
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Bibliographic Citation
- 한국미생물학회 프로시딩, pp.C004, 2005
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Publisher
- 한국미생물학회
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Type
- Conference
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Language
- English
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