해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성

Title
해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성
Alternative Title
Enantioselective Production of Levofloxacin by a Microbial Lipase : Screening and Characterization of the Lipase from the Yeast Yarrowia lipolytica 180
Author(s)
김준태; 서해점; 우정희; 강성균; 정병철; 이정현; 김상진
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2005-05-12
Abstract
Levofloxacin, (S)-isomer of the (R),(S)-ofloxacin, is a new fluoroquinolone antibiotic. To screen strains producing a lipase that hydrolyzes the (R),(S)-ofloxacin ester to levofloxacin, 151 isolates showing lipolytic activity on tributyrin agar plate were tested for the hydrolysis of ofloxacin ester. 3 strains among them were selected, and one of them, identified as the yeast Yarrowia lipolytica CL180, was found to enantioselectively hydrolyze the (S)-enantiomer of the (R),(S)-ofloxacin ester. The enantioselective lipase-encoding gene was cloned by screening genomic library of Y. lipolytica CL 180 and the sequence was determined. The lipase gene consisted of 1431-bp nucleotides, encoding a polypeptide of 476 amino acids with a molecular mass of 52 kDa. The consensus sequence G-X1-S-X2-G in most serine lipases was well conserved. The catalytic apparatus involving the triad serine, aspartate, and histidine was also found in the protein sequence. The amino acids comparison showed a high similarity with the yeast lipases of type B1 carboxylesterase/lipase family.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31482
Bibliographic Citation
한국미생물학회 프로시딩, pp.C004, 2005
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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