해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김준태 | - |
dc.contributor.author | 서해점 | - |
dc.contributor.author | 우정희 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 정병철 | - |
dc.contributor.author | 이정현 | - |
dc.contributor.author | 김상진 | - |
dc.date.accessioned | 2020-07-17T08:51:27Z | - |
dc.date.available | 2020-07-17T08:51:27Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2005-05-12 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/31482 | - |
dc.description.abstract | Levofloxacin, (S)-isomer of the (R),(S)-ofloxacin, is a new fluoroquinolone antibiotic. To screen strains producing a lipase that hydrolyzes the (R),(S)-ofloxacin ester to levofloxacin, 151 isolates showing lipolytic activity on tributyrin agar plate were tested for the hydrolysis of ofloxacin ester. 3 strains among them were selected, and one of them, identified as the yeast Yarrowia lipolytica CL180, was found to enantioselectively hydrolyze the (S)-enantiomer of the (R),(S)-ofloxacin ester. The enantioselective lipase-encoding gene was cloned by screening genomic library of Y. lipolytica CL 180 and the sequence was determined. The lipase gene consisted of 1431-bp nucleotides, encoding a polypeptide of 476 amino acids with a molecular mass of 52 kDa. The consensus sequence G-X1-S-X2-G in most serine lipases was well conserved. The catalytic apparatus involving the triad serine, aspartate, and histidine was also found in the protein sequence. The amino acids comparison showed a high similarity with the yeast lipases of type B1 carboxylesterase/lipase family. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | 한국미생물학회 프로시딩 | - |
dc.title | 해양미생물로부터 유래한 광학특이성 lipase를 이용한 Levofloxacin의 생성 | - |
dc.title.alternative | Enantioselective Production of Levofloxacin by a Microbial Lipase : Screening and Characterization of the Lipase from the Yeast Yarrowia lipolytica 180 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | C004 | - |
dc.citation.startPage | C004 | - |
dc.citation.title | 한국미생물학회 프로시딩 | - |
dc.contributor.alternativeName | 김준태 | - |
dc.contributor.alternativeName | 우정희 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | 한국미생물학회 프로시딩, pp.C004 | - |
dc.description.journalClass | 2 | - |