메타게노믹스를 이용한 해양환경유래 지질분해효소 탐색

Title
메타게노믹스를 이용한 해양환경유래 지질분해효소 탐색
Alternative Title
SCREENING LIPOLYTIC ENZYMES FROM VARIOUS MARINE ENVIRONMENTS BY METAGENOMIC APPROACH
Author(s)
전정호; 임재규; 김준태; 이현숙; 강성균; 김상진; 이정현
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2007-03-11
Abstract
Metagenomic libraries of various marine samples collected from a seamount near hydrothermal vent, Artic coast (Dasan station) and inter tidal flat of Kangwha island were constructed using a fosmid vector. The total size of the metagenomic library was estimated to about 8 Gb (more than 200,000 clones). The library was screened for the lipolytic activity by plating the clones on TBN plate, and as a result, eighteen positive clones forming a halo were obtained. The genes representing the lipolytic activities were cloned. The sequence analysis revealed that all of 18 genes contained an oxyanion hole (XG) and an active-site (GXSXG) consensus region, and showed a significant similarity to α/β hydrolase family, essentially esterase/lipases. In an attempt to express the esterase/lipase genes in E. coli, most of those enzymes were not produced in a soluble form. Several approaches to resolve the solubility issue are under progress.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30652
Bibliographic Citation
8-th International Marine Biotechnology Conference, pp.1, 2007
Publisher
8-th International Marine Biotechnology Conference
Type
Conference
Language
English
Publisher
8-th International Marine Biotechnology Conference
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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