메타게노믹스를 이용한 해양환경유래 지질분해효소 탐색
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전정호 | - |
dc.contributor.author | 임재규 | - |
dc.contributor.author | 김준태 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 김상진 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T04:50:40Z | - |
dc.date.available | 2020-07-17T04:50:40Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2007-03-11 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/30652 | - |
dc.description.abstract | Metagenomic libraries of various marine samples collected from a seamount near hydrothermal vent, Artic coast (Dasan station) and inter tidal flat of Kangwha island were constructed using a fosmid vector. The total size of the metagenomic library was estimated to about 8 Gb (more than 200,000 clones). The library was screened for the lipolytic activity by plating the clones on TBN plate, and as a result, eighteen positive clones forming a halo were obtained. The genes representing the lipolytic activities were cloned. The sequence analysis revealed that all of 18 genes contained an oxyanion hole (XG) and an active-site (GXSXG) consensus region, and showed a significant similarity to α/β hydrolase family, essentially esterase/lipases. In an attempt to express the esterase/lipase genes in E. coli, most of those enzymes were not produced in a soluble form. Several approaches to resolve the solubility issue are under progress. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 8-th International Marine Biotechnology Conference | - |
dc.relation.isPartOf | 8-th International Marine Biotechnology Conference | - |
dc.title | 메타게노믹스를 이용한 해양환경유래 지질분해효소 탐색 | - |
dc.title.alternative | SCREENING LIPOLYTIC ENZYMES FROM VARIOUS MARINE ENVIRONMENTS BY METAGENOMIC APPROACH | - |
dc.type | Conference | - |
dc.citation.endPage | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | 8-th International Marine Biotechnology Conference | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 김준태 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 8-th International Marine Biotechnology Conference, pp.1 | - |
dc.description.journalClass | 1 | - |