한국 동해산 해양생물의 종 동정을 위한 DNA바코드-DNA칩 개발

Abstract

As a tool for rapid identification of marine organisms, a DNA chip with oligonucleotide probes were developed based on the DNA barcode information. Approximately 650 nucleotide long DNA barcode sequences were obtained from Osteichthyes (Scomber japonicus, Gadus macrocepalus, Stephanolepis cirrhifer, Thamnaconus modestus), Molluscus (Yodarodes pacificus, Batillus cornutus, Pomaulax japonicus, Croassostrea nippona, Crossostera gigas), and Crustaceans (Chionoecetes opilio, Chionoecetes japonica) which are some of the dominant fisheries species in the East Sre of Korea. The sequences were compared with those of congeneric species deposited in the GenBank and species specific nucleotides were identified. Then, 21 to 33 nucleotide long probes were designed, synthesized, and spotted on a glass slide. To test the usefulness of the DNA chip, fragments of the COI gene were amplified from marine samples and hybridized with the probes. Distinct hybridization patterns appeared on the DNA chip dependent on the species. Such DNA chips were also made for Salmonid and Rajiformes fishes and tested useful. As one of the utilizations of the DNA barcode information, the DNA chip method provides a rapid, reliable, and objective measure for identification of marine organisms of interest.