한국 동해산 해양생물의 종 동정을 위한 DNA바코드-DNA칩 개발

Title
한국 동해산 해양생물의 종 동정을 위한 DNA바코드-DNA칩 개발
Alternative Title
Development of A DNA Barcode-based DNA chip Identification of marine organisms in the East Sea of Korea
Author(s)
이윤호; 정다금; 김고은; 김충곤; 박흥식; 배세진; 정진욱; 윤현규; 황승용
KIOST Author(s)
Lee, Youn Ho(이윤호)Kim, Choong Gon(김충곤)Park, Heung-Sik(박흥식)
Publication Year
2007-09-18
Abstract
As a tool for rapid identification of marine organisms, a DNA chip with oligonucleotide probes were developed based on the DNA barcode information. Approximately 650 nucleotide long DNA barcode sequences were obtained from Osteichthyes (Scomber japonicus, Gadus macrocepalus, Stephanolepis cirrhifer, Thamnaconus modestus), Molluscus (Yodarodes pacificus, Batillus cornutus, Pomaulax japonicus, Croassostrea nippona, Crossostera gigas), and Crustaceans (Chionoecetes opilio, Chionoecetes japonica) which are some of the dominant fisheries species in the East Sre of Korea. The sequences were compared with those of congeneric species deposited in the GenBank and species specific nucleotides were identified. Then, 21 to 33 nucleotide long probes were designed, synthesized, and spotted on a glass slide. To test the usefulness of the DNA chip, fragments of the COI gene were amplified from marine samples and hybridized with the probes. Distinct hybridization patterns appeared on the DNA chip dependent on the species. Such DNA chips were also made for Salmonid and Rajiformes fishes and tested useful. As one of the utilizations of the DNA barcode information, the DNA chip method provides a rapid, reliable, and objective measure for identification of marine organisms of interest.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30364
Bibliographic Citation
2nd International Barcode of Life Conference, pp.23, 2007
Publisher
국제바코드컨소시엄
Type
Conference
Language
English
Publisher
국제바코드컨소시엄
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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