한국 동해산 해양생물의 종 동정을 위한 DNA바코드-DNA칩 개발

DC Field Value Language
dc.contributor.author 이윤호 -
dc.contributor.author 정다금 -
dc.contributor.author 김고은 -
dc.contributor.author 김충곤 -
dc.contributor.author 박흥식 -
dc.contributor.author 배세진 -
dc.contributor.author 정진욱 -
dc.contributor.author 윤현규 -
dc.contributor.author 황승용 -
dc.date.accessioned 2020-07-17T03:31:17Z -
dc.date.available 2020-07-17T03:31:17Z -
dc.date.created 2020-02-11 -
dc.date.issued 2007-09-18 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/30364 -
dc.description.abstract As a tool for rapid identification of marine organisms, a DNA chip with oligonucleotide probes were developed based on the DNA barcode information. Approximately 650 nucleotide long DNA barcode sequences were obtained from Osteichthyes (Scomber japonicus, Gadus macrocepalus, Stephanolepis cirrhifer, Thamnaconus modestus), Molluscus (Yodarodes pacificus, Batillus cornutus, Pomaulax japonicus, Croassostrea nippona, Crossostera gigas), and Crustaceans (Chionoecetes opilio, Chionoecetes japonica) which are some of the dominant fisheries species in the East Sre of Korea. The sequences were compared with those of congeneric species deposited in the GenBank and species specific nucleotides were identified. Then, 21 to 33 nucleotide long probes were designed, synthesized, and spotted on a glass slide. To test the usefulness of the DNA chip, fragments of the COI gene were amplified from marine samples and hybridized with the probes. Distinct hybridization patterns appeared on the DNA chip dependent on the species. Such DNA chips were also made for Salmonid and Rajiformes fishes and tested useful. As one of the utilizations of the DNA barcode information, the DNA chip method provides a rapid, reliable, and objective measure for identification of marine organisms of interest. -
dc.description.uri 1 -
dc.language English -
dc.publisher 국제바코드컨소시엄 -
dc.relation.isPartOf 2nd International Barcode of Life Conference -
dc.title 한국 동해산 해양생물의 종 동정을 위한 DNA바코드-DNA칩 개발 -
dc.title.alternative Development of A DNA Barcode-based DNA chip Identification of marine organisms in the East Sea of Korea -
dc.type Conference -
dc.citation.endPage 23 -
dc.citation.startPage 23 -
dc.citation.title 2nd International Barcode of Life Conference -
dc.contributor.alternativeName 이윤호 -
dc.contributor.alternativeName 정다금 -
dc.contributor.alternativeName 김고은 -
dc.contributor.alternativeName 김충곤 -
dc.contributor.alternativeName 박흥식 -
dc.contributor.alternativeName 배세진 -
dc.identifier.bibliographicCitation 2nd International Barcode of Life Conference, pp.23 -
dc.description.journalClass 1 -
Appears in Collections:
Ocean Climate Solutions Research Division > Ocean Climate Response & Ecosystem Research Department > 2. Conference Papers
Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 2. Conference Papers
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