유전체를 이용한 해양미생물로부터 광학특이적 epoxide hydrolase개발

Title
유전체를 이용한 해양미생물로부터 광학특이적 epoxide hydrolase개발
Alternative Title
Development of enantioselective epoxide hydrolase activities from marine microorganisms using genomic approach
Author(s)
강성균; 황영옥; 우정희; 강지현; 김상진
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2007-09-23
Abstract
To develop epoxide hydrolases from marine microorganisms, genomic information of marine microorganisms has been analyzed using various bioinformatic tools. Three genes encoding putative EHases were identified by analyzing open reading frames (ORFs) of Erythrobacter litoralis HTCC2594. Despite low similarities to reported EHases, the phylogenetic analysis of the three genes showed that eeh1 was similar to microsomal EHase while eeh2 and eeh3 could be grouped with soluble EHases. In addition, genes encoding putative EHases were also identified by analyzing open reading frames (ORFs) of several marine microorganisms whose genome sequences are available in public. Three additional EHases - sEEH, nEEH, and rEEH – from marine microorganisms could be found. The recombinant EHases overexpressed in E. coli were purified by metal affinity chromatography and further characterized. The application to chiral resolution of racemic epoxide substrates by purified enzymes is under progress.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30355
Bibliographic Citation
제19회 Enzyme Engineering, pp.1, 2007
Publisher
Engineering Conference International
Type
Conference
Language
English
Publisher
Engineering Conference International
Related Researcher
Research Interests

marine biotechnology,microbiology,해양생명공학,미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse