유전체를 이용한 해양미생물로부터 광학특이적 epoxide hydrolase개발

Abstract

To develop epoxide hydrolases from marine microorganisms, genomic information of marine microorganisms has been analyzed using various bioinformatic tools. Three genes encoding putative EHases were identified by analyzing open reading frames (ORFs) of Erythrobacter litoralis HTCC2594. Despite low similarities to reported EHases, the phylogenetic analysis of the three genes showed that eeh1 was similar to microsomal EHase while eeh2 and eeh3 could be grouped with soluble EHases. In addition, genes encoding putative EHases were also identified by analyzing open reading frames (ORFs) of several marine microorganisms whose genome sequences are available in public. Three additional EHases - sEEH, nEEH, and rEEH – from marine microorganisms could be found. The recombinant EHases overexpressed in E. coli were purified by metal affinity chromatography and further characterized. The application to chiral resolution of racemic epoxide substrates by purified enzymes is under progress.