Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay
Author(s)
Song, Yong Bhum; Park, Chul O.; Jeong, Jae-Yeon; Huh, Won-Ki
Publication Year
2014-03-15
Abstract
G protein-coupled receptors (GPCRs) are the largest family of cell-surface receptors and are involved in a variety of pathological conditions including cancer and cardiovascular, metabolic, neurological, and autoimmune diseases. GPCRs are being intensively investigated as targets for therapeutic intervention, and the beta-arrestin recruitment assay has become a popular tool for analyzing GPCR activation. Here, we report a high-throughput method for cloning GPCR cDNAs into adenoviral bimolecular fluorescence complementation (BiFC) vectors and performing the beta-arrestin BiFC assay in cells transduced with recombinant adenoviruses. An analysis of the activation of somatostatin receptor 2 (SSTR2) with the adenovirus-based beta-arrestin BiFC assay showed that the assay is suitable for quantifying SSTR2 activation in response to specific agonists or antagonists. Furthermore, the adenovirus-based beta-arrestin BiFC assay was able to detect the activation of a broad range of GPCRs. Collectively, our data indicate that the adenovirus-based beta-arrestin BiFC assay can serve as a simple and universal platform for studying GPCR activation and thus will be useful for high-throughput screening of drugs that target GPCRs. (C) 2013 Elsevier Inc. All rights reserved.
ISSN
0003-2697
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2891
DOI
10.1016/j.ab.2013.12.017
Bibliographic Citation
ANALYTICAL BIOCHEMISTRY, v.449, pp.32 - 41, 2014
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Subject
DRUG DISCOVERY; 7-TRANSMEMBRANE RECEPTORS; CELLS; BETA-ARRESTIN2; VISUALIZATION; TRAFFICKING; DEGRADATION; ENDOCYTOSIS; COMPLEXES
Keywords
G protein-coupled receptor (GPCR); beta-Arrestin; Bimolecular fluorescence complementation (BiFC); Adenovirus; High-throughput screening
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
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