Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Song, Yong Bhum | - |
dc.contributor.author | Park, Chul O. | - |
dc.contributor.author | Jeong, Jae-Yeon | - |
dc.contributor.author | Huh, Won-Ki | - |
dc.date.accessioned | 2020-04-20T04:55:06Z | - |
dc.date.available | 2020-04-20T04:55:06Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014-03-15 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2891 | - |
dc.description.abstract | G protein-coupled receptors (GPCRs) are the largest family of cell-surface receptors and are involved in a variety of pathological conditions including cancer and cardiovascular, metabolic, neurological, and autoimmune diseases. GPCRs are being intensively investigated as targets for therapeutic intervention, and the beta-arrestin recruitment assay has become a popular tool for analyzing GPCR activation. Here, we report a high-throughput method for cloning GPCR cDNAs into adenoviral bimolecular fluorescence complementation (BiFC) vectors and performing the beta-arrestin BiFC assay in cells transduced with recombinant adenoviruses. An analysis of the activation of somatostatin receptor 2 (SSTR2) with the adenovirus-based beta-arrestin BiFC assay showed that the assay is suitable for quantifying SSTR2 activation in response to specific agonists or antagonists. Furthermore, the adenovirus-based beta-arrestin BiFC assay was able to detect the activation of a broad range of GPCRs. Collectively, our data indicate that the adenovirus-based beta-arrestin BiFC assay can serve as a simple and universal platform for studying GPCR activation and thus will be useful for high-throughput screening of drugs that target GPCRs. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | DRUG DISCOVERY | - |
dc.subject | 7-TRANSMEMBRANE RECEPTORS | - |
dc.subject | CELLS | - |
dc.subject | BETA-ARRESTIN2 | - |
dc.subject | VISUALIZATION | - |
dc.subject | TRAFFICKING | - |
dc.subject | DEGRADATION | - |
dc.subject | ENDOCYTOSIS | - |
dc.subject | COMPLEXES | - |
dc.title | Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay | - |
dc.type | Article | - |
dc.citation.endPage | 41 | - |
dc.citation.startPage | 32 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 449 | - |
dc.contributor.alternativeName | 정재연 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.449, pp.32 - 41 | - |
dc.identifier.doi | 10.1016/j.ab.2013.12.017 | - |
dc.identifier.scopusid | 2-s2.0-84892727968 | - |
dc.identifier.wosid | 000332816100005 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | DRUG DISCOVERY | - |
dc.subject.keywordPlus | 7-TRANSMEMBRANE RECEPTORS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | BETA-ARRESTIN2 | - |
dc.subject.keywordPlus | VISUALIZATION | - |
dc.subject.keywordPlus | TRAFFICKING | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordAuthor | G protein-coupled receptor (GPCR) | - |
dc.subject.keywordAuthor | beta-Arrestin | - |
dc.subject.keywordAuthor | Bimolecular fluorescence complementation (BiFC) | - |
dc.subject.keywordAuthor | Adenovirus | - |
dc.subject.keywordAuthor | High-throughput screening | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |