Screening of beta-glucosidase, beta-glucanase, xylanase and agarase producing marine microbes and amplification of the partial genes

Title
Screening of beta-glucosidase, beta-glucanase, xylanase and agarase producing marine microbes and amplification of the partial genes
Author(s)
오철홍; 허수진; Abu Affan; 박흥식; 권영경; 전선미; 예보람; 김태호; 강도형
KIOST Author(s)
Oh, Chulhong(오철홍)Heo, Soo Jin(허수진)Park, Heung-Sik(박흥식)Kim, Taeho(김태호)Kang, Do-Hyung(강도형)
Publication Year
2010-11-18
Abstract
Saccharification enzymes are very useful for industry as bioethanol production, functional food and cosmetic. We tried for find relative genes from marine microbes. Samples were collected from coast of Chuuk state (Micronesia) and Jeju (Republic of Korea) for isolate of saccharification enzyme producing bacteria. The enzymes were targeted as beta-glucosidase, beta-1,4-glucanase, beta-1,3-glucanase, beta-1,4-xylanase and agarase. Isolated strains were identified by 16s rRNA sequences. Based on the identified bacteria, primers were designed by compare with targeted genes of relative strains to genus or family or order from NCBI database. Genomic DNAs were isolated from each strain, and PCR were performed with different conditions. The PCR products were cloned into pGEM easy T vector and analyzed the sequences with nucleotide Blast and BlastX programs. According to sequencing results, we identified 33 different nucleotide sequences of partial or coding including beta-glucanase of seven, beta-glucosidase of twenty, xylanase of five and agarase of one. Almost genes were novel sequences with 40~90% homologues. We are going to study for finding full-length genes, and develop effective over-expression and purification system.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28522
Bibliographic Citation
The 6th KSMB Annual Meeting & International Mini-symposium, 2010, pp.105, 2010
Publisher
Korean Society of Marine Biotechnology
Type
Conference
Language
English
Publisher
Korean Society of Marine Biotechnology
Related Researcher
Research Interests

Marine ecology,Marine biology,Marine living resources,해양생태,해양생물 생리,해양생물자원

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