Screening of beta-glucosidase, beta-glucanase, xylanase and agarase producing marine microbes and amplification of the partial genes

Abstract

Saccharification enzymes are very useful for industry as bioethanol production, functional food and cosmetic. We tried for find relative genes from marine microbes. Samples were collected from coast of Chuuk state (Micronesia) and Jeju (Republic of Korea) for isolate of saccharification enzyme producing bacteria. The enzymes were targeted as beta-glucosidase, beta-1,4-glucanase, beta-1,3-glucanase, beta-1,4-xylanase and agarase. Isolated strains were identified by 16s rRNA sequences. Based on the identified bacteria, primers were designed by compare with targeted genes of relative strains to genus or family or order from NCBI database. Genomic DNAs were isolated from each strain, and PCR were performed with different conditions. The PCR products were cloned into pGEM easy T vector and analyzed the sequences with nucleotide Blast and BlastX programs. According to sequencing results, we identified 33 different nucleotide sequences of partial or coding including beta-glucanase of seven, beta-glucosidase of twenty, xylanase of five and agarase of one. Almost genes were novel sequences with 40~90% homologues. We are going to study for finding full-length genes, and develop effective over-expression and purification system.