축라군에서 barcoded pyrosequencing을 사용한 초미소생물 다양성

Abstract

To elucidate spatial changes of picocyanobacterial genetic diversity in the Chuuk Lagoon, Federated States of Micronesia (FSM), a high-throughput bar-coded pyrosequencing technique was used for 16S-23S internal transcribed spacer (ITS) sequences. The samples were collected from 2water masses, open lagoon water and water adjacent to mangroves. From 33 samples, partial ITS sequences were amplified by PCR using each bar-coded primers, and then sequenced by pyrosequencing method (GSFLX Titanium). A total of 57,600 sequences were obtained, and classified into 16Synechococcus and 3 Prochlorococcus clades.For the lagoon waters, Synechococcus clade II sequences were dominant followed by clades III, VII, IX, X. For Prochlorococcus, clade HLII was the dominant sequence and low-light adapted clades were not found in our samples. On the other hand, mangrove water samples showed distinct spatial differences. Mangrove waters showed no Prochlorococcussequences. For Synechococcus, the dominant sequences were clade II and CB5. Going further away from the mangroves, there were a decreasing trend in Synechococcus CB5 sequences but an increasing trend for Prochlorococcus clade HLII. Sequence composition similar to the open lagoon water only started to appear from the sampling station located about 1 km away from the mangroves. These results suggest that habitat characteristics of the tropical lagoon drive varsequences. The samples were collected from 2water masses, open lagoon water and water adjacent to mangroves. From 33 samples, partial ITS sequences were amplified by PCR using each bar-coded primers, and then sequenced by pyrosequencing method (GSFLX Titanium). A total of 57,600 sequences were obtained, and classified into 16Synechococcus and 3 Prochlorococcus clades.For the lagoon waters, Synechococcus clade II sequences were dominant followed by clades III, VII, IX, X. For Prochlorococcus, clade HLII was the dominant sequence and low-light