Morphological Characteristic and Cellular components of Newly Isolated Myxosarcina sp. KORDI-1 from Mangrove forest in Chuuk State, Federated States of Micronesia

Abstract

We isolated a cyanobacterium from root sediment of mangrove forest in Chuuk State, Federated States of Micronesia, and then identified it as belonging to the genus Myxosarcina (strain designated Myxosarcina sp. KORDI-5). In the morphological analysis using LM and SEM, the periphytic cyanobacterial cells were rounded cubes to spherical with yellow-brown or grayish, and the colonies were densely packed 4-64 non-polarized blastoparenchymatous cells. Ultrastructural analysis showed progress of cell division in the inner sheath, and the colonies cells possessed complex sheaths. In order to evaluate its potential use of industrial application, cellular components of the strain were further analyzed. According to the biochemical analysis of the strain, its carbohydrate (31.5±0.1%), protein (19.4±0.1%), lipid (18.8±0.4%), ash (27.7±0.5) and moisture (2.7±0.1%) contents were estimated. Moreover, we examined the compositions of amino acids, monosaccarides and fatty acids in Myxosarcina sp. KORDI-1. The dominant amino acid was glutamic acid (12.68%), while D-glucose (46.8%) was dominant in monosaccarides. Also, oleic acid (59.7%) was dominantly found from in it. Based on these results, the newly isolated Myxosarcina sp. KORDI-1 will have a potential for the industrial use in the future microalgal biotechnologyl analysis using LM and SEM, the periphytic cyanobacterial cells were rounded cubes to spherical with yellow-brown or grayish, and the colonies were densely packed 4-64 non-polarized blastoparenchymatous cells. Ultrastructural analysis showed progress of cell division in the inner sheath, and the colonies cells possessed complex sheaths. In order to evaluate its potential use of industrial application, cellular components of the strain were further analyzed. According to the biochemical analysis of the strain, its carbohydrate (31.5±0.1%), protein (19.4±0.1%), lipid (18.8±0.4%), ash (27.7±0.5) and moisture (2.7±0.1%) contents were estimated. Moreover, we examined the compositions of amino acids, monosaccarides and fatty acids in Myxosarcina sp. KORDI-1. The dominant amino acid was glutamic acid (12.68%), while D-glucose (46.8%) was dominant in monosaccarides. Also, oleic acid (59.7%) was dominantly found from in it. Based on these results, the newly isolated Myxosarcina sp. KORDI-1 will have a potential for the industrial use in the future microalgal biotechnology