Enhancement of FAMEs Compositions of Tetraselmis sp. YH by Modified Media using Natural Resources

Title
Enhancement of FAMEs Compositions of Tetraselmis sp. YH by Modified Media using Natural Resources
Author(s)
최우석; 김태호; 이경아; 박아름이; 강도형
KIOST Author(s)
Kim, Taeho(김태호)Park, Areumi(박아름이)Kang, Do-Hyung(강도형)
Publication Year
2016-11-25
Abstract
The aim of this study is to enhance the FAMEs compositions of Tetraselmis sp. YH using by modified media using natural resources. The following culture experimental design was used to evaluate the effects of natural resources: each experimental group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of natural resources in the laboratory photobioreactor culture system (10-days culture). Then analyzed cell counting and FAMEs compositions using FACs and GC/MS methods, respectively. In an experiment using OF, biomass production (0.91±0.03 g/L) of C-group experiment (a ratio of f/2:OF=31:1) was higher than that of control-group (0.56±0.02 g/L). Especially, the total FAMEs compositions of C-group were recorded to 79.59 mg/g. In an experiment using ME, biomass production (0.89±0.05 g/L) of B-group (a ratio of f/2:ME=15:1) was higher than that of control-group (0.56±0.02 g/L) while FAMEs compositions were 83.17 mg/g, 76.07 mg/g, respectively. In an experiment using the cocktail of OF and ME, biomass production (1.04±0.03 g/L) of A-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.56±0.02 g/L). Particularly, FAMEs contents were highest of 84.44 mg/g than all of experimental groups. We suggested that Tetraselmis sp. YH had a higher potential for producing highesntal group was made from modified Guillards f/2 medium with mud extract (ME) by autoclave and organic fertilizer (OF). Furthermore, A- to D-group (except control) was divided by concentrations of natural resources in the laboratory photobioreactor culture system (10-days culture). Then analyzed cell counting and FAMEs compositions using FACs and GC/MS methods, respectively. In an experiment using OF, biomass production (0.91±0.03 g/L) of C-group experiment (a ratio of f/2:OF=31:1) was higher than that of control-group (0.56±0.02 g/L). Especially, the total FAMEs compositions of C-group were recorded to 79.59 mg/g. In an experiment using ME, biomass production (0.89±0.05 g/L) of B-group (a ratio of f/2:ME=15:1) was higher than that of control-group (0.56±0.02 g/L) while FAMEs compositions were 83.17 mg/g, 76.07 mg/g, respectively. In an experiment using the cocktail of OF and ME, biomass production (1.04±0.03 g/L) of A-group (a ratio of f/2:OF:ME=8:1:7) was highest than that of control-group (0.56±0.02 g/L). Particularly, FAMEs contents were highest of 84.44 mg/g than all of experimental groups. We suggested that Tetraselmis sp. YH had a higher potential for producing highes
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24300
Bibliographic Citation
2016년 한국해양바이오학회 정기총회 및 학술발표회, pp.128 - 129, 2016
Publisher
한국해양바이오학회
Type
Conference
Language
English
Publisher
한국해양바이오학회
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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