표면반응응답법을 이용한 해수배지에서 Thermococcus onnurineus NA1의 수소생산 최적화

Title
표면반응응답법을 이용한 해수배지에서 Thermococcus onnurineus NA1의 수소생산 최적화
Alternative Title
Optimization of hydrogen production of Thermococcus onnurineus NA1 in a seawater medium by using response surface methodology
Author(s)
김태완; 김재영; 이현숙; 이정현; 강성균
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2018-06-27
Abstract
The hyperthermophilic archaeon, Thermococcus onnurineus NA1, isolated from the deep sea hydrothermal area, produces hydrogen while growing on a medium with carbon monoxide (CO) at head space. In the present study, we attempted to develop aseawater (SW) medium and optimize its culture condition by employing response surface methodology (RSM) to enhance H2 production by T. onnurineus NA1 because a previous medium composed of artificial seawater required various chemical salt supplementation. It was found that sea water could substitute artificial sea water with comparable hydrogen production to that of MM1. Then, the effects of major components of MM1 medium such as the yeast extract, FeSO4, EDTA, trace elements, vitamins, and cystein-HCl were determined in the sea water condition. Additionally, culture condition was also optimized by RSM and 506.12 mmol/L/h hydrogen production could be predicted by the resulting optimization numerical solution. Finally, the maximum hydrogen production of 497.74 mmol/L/h could be experimentally demonstrated in a SW medium composed of 14.53 g/L of yeast extract with CO rate and agitation amounting to 0.25 vvm and 980 rpm, respectively.eawater (SW) medium and optimize its culture condition by employing response surface methodology (RSM) to enhance H2 production by T. onnurineus NA1 because a previous medium composed of artificial seawater required various chemical salt supplementation. It was found that sea water could substitute artificial sea water with comparable hydrogen production to that of MM1. Then, the effects of major components of MM1 medium such as the yeast extract, FeSO4, EDTA, trace elements, vitamins, and cystein-HCl were determined in the sea water condition. Additionally, culture condition was also optimized by RSM and 506.12 mmol/L/h hydrogen production could be predicted by the resulting optimization numerical solution. Finally, the maximum hydrogen production of 497.74 mmol/L/h could be experimentally demonstrated in a SW medium composed of 14.53 g/L of yeast extract with CO rate and agitation amounting to 0.25 vvm and 980 rpm, respectively.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23198
Bibliographic Citation
2018년 국제학술대회 및 정기학술대회, pp.38, 2018
Publisher
한국미생물
Type
Conference
Language
English
Publisher
한국미생물
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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