표면반응응답법을 이용한 해수배지에서 Thermococcus onnurineus NA1의 수소생산 최적화

Abstract

The hyperthermophilic archaeon, Thermococcus onnurineus NA1, isolated from the deep sea hydrothermal area, produces hydrogen while growing on a medium with carbon monoxide (CO) at head space. In the present study, we attempted to develop aseawater (SW) medium and optimize its culture condition by employing response surface methodology (RSM) to enhance H2 production by T. onnurineus NA1 because a previous medium composed of artificial seawater required various chemical salt supplementation. It was found that sea water could substitute artificial sea water with comparable hydrogen production to that of MM1. Then, the effects of major components of MM1 medium such as the yeast extract, FeSO4, EDTA, trace elements, vitamins, and cystein-HCl were determined in the sea water condition. Additionally, culture condition was also optimized by RSM and 506.12 mmol/L/h hydrogen production could be predicted by the resulting optimization numerical solution. Finally, the maximum hydrogen production of 497.74 mmol/L/h could be experimentally demonstrated in a SW medium composed of 14.53 g/L of yeast extract with CO rate and agitation amounting to 0.25 vvm and 980 rpm, respectively.eawater (SW) medium and optimize its culture condition by employing response surface methodology (RSM) to enhance H2 production by T. onnurineus NA1 because a previous medium composed of artificial seawater required various chemical salt supplementation. It was found that sea water could substitute artificial sea water with comparable hydrogen production to that of MM1. Then, the effects of major components of MM1 medium such as the yeast extract, FeSO4, EDTA, trace elements, vitamins, and cystein-HCl were determined in the sea water condition. Additionally, culture condition was also optimized by RSM and 506.12 mmol/L/h hydrogen production could be predicted by the resulting optimization numerical solution. Finally, the maximum hydrogen production of 497.74 mmol/L/h could be experimentally demonstrated in a SW medium composed of 14.53 g/L of yeast extract with CO rate and agitation amounting to 0.25 vvm and 980 rpm, respectively.