Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans SCIE SCOPUS KCI

Cited 4 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans
Author(s)
An, Young Jun; Rowland, Sara E.; Robb, Frank T.; Cha, Sun-Shin
KIOST Author(s)
Young, Jun An(안영준)
Publication Year
2016-06
Abstract
Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 angstrom resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 angstrom resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2183
DOI
10.1007/s12275-016-6089-5
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.54, no.6, pp.440 - 444, 2016
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
COMPLEX; GENE
Keywords
Group III chaperonins; protein folding; ATP-fueled nanomachine; crystallization
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

Structural biology,Protein Engineering,구조생물학,단백질 공학

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