Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans
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Title
- Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans
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Author(s)
- An, Young Jun; Rowland, Sara E.; Robb, Frank T.; Cha, Sun-Shin
- KIOST Author(s)
- An, Young Jun(안영준)
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Alternative Author(s)
- 안영준
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Publication Year
- 2016-06
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Abstract
- Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 angstrom resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 angstrom resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%.
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ISSN
- 1225-8873
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2183
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DOI
- 10.1007/s12275-016-6089-5
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Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.54, no.6, pp.440 - 444, 2016
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Publisher
- MICROBIOLOGICAL SOCIETY KOREA
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Subject
- COMPLEX; GENE
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Keywords
- Group III chaperonins; protein folding; ATP-fueled nanomachine; crystallization
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Type
- Article
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Language
- English
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Document Type
- Article
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