Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | An, Young Jun | - |
dc.contributor.author | Rowland, Sara E. | - |
dc.contributor.author | Robb, Frank T. | - |
dc.contributor.author | Cha, Sun-Shin | - |
dc.date.accessioned | 2020-04-20T02:40:04Z | - |
dc.date.available | 2020-04-20T02:40:04Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2183 | - |
dc.description.abstract | Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 angstrom resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 angstrom resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | COMPLEX | - |
dc.subject | GENE | - |
dc.title | Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans | - |
dc.type | Article | - |
dc.citation.endPage | 444 | - |
dc.citation.startPage | 440 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 54 | - |
dc.citation.number | 6 | - |
dc.contributor.alternativeName | 안영준 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.54, no.6, pp.440 - 444 | - |
dc.identifier.doi | 10.1007/s12275-016-6089-5 | - |
dc.identifier.scopusid | 2-s2.0-84969919749 | - |
dc.identifier.wosid | 000376653000006 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Group III chaperonins | - |
dc.subject.keywordAuthor | protein folding | - |
dc.subject.keywordAuthor | ATP-fueled nanomachine | - |
dc.subject.keywordAuthor | crystallization | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |