수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법

Title
수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법
Alternative Title
a genetically Engineered hyperthermophilic microoganism with an enhanced WGS activity
Author(s)
이정현; 강성균; 이현숙; 권개경; 배승섭; 이성혁; 임재규; 김민식; 김태완; 김윤재
KIOST Author(s)
Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)Kwon, Kae Kyoung(권개경)
Application Date
2012-06-19
Registration Date
2014-05-23
Application No.
10-2012-0065303 KIPRIS
Registration No.
10-1401603
Country
KO
Abstract
Thermococcus onnurineus NA1 is capable of producing hydrogen under anaerobic growth conditions
supplemented with either CO, formate, or starch. Herein, we describe that the carboxydotrophic
hydrogenogenic activity (CO+H2O→CO2+H2) in the strain could be explained by the presence of a
gene cluster encoding a monofunctional CO dehydrogenase (codh) and a membrance bound hydrogenase
(mch). The strain was highly insentive to 100% CO, maintaining the carboxydotrophic hydrogenogenic
growth above 2 ATM of 100% CO. The hydrogen productivity from CO was enhanced significantly by the
modification of the promoter region of the carbon monoxide dehydrogenase, well-correlated with the
enhanced expression level of the genes in the codh-mch gene cluster by RT-PCR and western
blotting. The mutant, designated as MC01, produced 50~120 mmol hydrogen/l/h, compared to 15~30mmol
hydrogen/l/h of wild-type strain while the conversion yield from CO to hydrogen in the mutant
strain was similar to that of wild-type strain. In a practical perspective, the MC01 strain was
applied to hydrogen production using by-product gases of steel-making company, resulting in 58
mmol/l/h productivity from LDG furnace gas. This work represents that the combinatorial approach
of genomic analysis and conventional genetic engineering by modulating the expression of the
carbon monoxide hydrogen lyase (codh-mch) was quite effective in prod
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/21441
Type
Patent
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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