수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법

DC Field Value Language
dc.contributor.author 이정현 -
dc.contributor.author 강성균 -
dc.contributor.author 이현숙 -
dc.contributor.author 권개경 -
dc.contributor.author 배승섭 -
dc.contributor.author 이성혁 -
dc.contributor.author 임재규 -
dc.contributor.author 김민식 -
dc.contributor.author 김태완 -
dc.contributor.author 김윤재 -
dc.date.accessioned 2020-07-15T06:07:05Z -
dc.date.available 2020-07-15T06:07:05Z -
dc.date.issued 2012-06-19 -
dc.date.issued 2014-05-23 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/21441 -
dc.description.abstract Thermococcus onnurineus NA1 is capable of producing hydrogen under anaerobic growth conditions supplemented with either CO, formate, or starch. Herein, we describe that the carboxydotrophic hydrogenogenic activity (CO+H2O→CO2+H2) in the strain could be explained by the presence of a gene cluster encoding a monofunctional CO dehydrogenase (codh) and a membrance bound hydrogenase (mch). The strain was highly insentive to 100% CO, maintaining the carboxydotrophic hydrogenogenic growth above 2 ATM of 100% CO. The hydrogen productivity from CO was enhanced significantly by the modification of the promoter region of the carbon monoxide dehydrogenase, well-correlated with the enhanced expression level of the genes in the codh-mch gene cluster by RT-PCR and western blotting. The mutant, designated as MC01, produced 50~120 mmol hydrogen/l/h, compared to 15~30mmol hydrogen/l/h of wild-type strain while the conversion yield from CO to hydrogen in the mutant strain was similar to that of wild-type strain. In a practical perspective, the MC01 strain was applied to hydrogen production using by-product gases of steel-making company, resulting in 58 mmol/l/h productivity from LDG furnace gas. This work represents that the combinatorial approach of genomic analysis and conventional genetic engineering by modulating the expression of the carbon monoxide hydrogen lyase (codh-mch) was quite effective in prod -
dc.title 수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법 -
dc.title.alternative a genetically Engineered hyperthermophilic microoganism with an enhanced WGS activity -
dc.type Patent -
dc.date.application 2012-06-19 -
dc.date.registration 2014-05-23 -
dc.identifier.patentApplicationNumber 10-2012-0065303 -
dc.identifier.patentRegistrationNumber 10-1401603 -
dc.type.iprs 특허 -
dc.publisher.country KO -
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Marine Resources Research Division > Marine Biotechnology Research Center > 4. Patents
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