Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection SCIE SCOPUS

Cited 26 time in WEB OF SCIENCE Cited 26 time in Scopus
Title
Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection
Author(s)
Nikapitiya, Chamilani; Dananjaya, S. H. S.; De Silva, B. C. J.; Heo, Gang-Joon; Oh, Chulhong; De Zoysa, Mahanama; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)
Publication Year
2018-05
Abstract
Chitosan nanoparticles (CNPs) were synthesized by ionic gelation method and its immunomodulatory properties were investigated in zebrafish larvae. Average particle size and zeta potential were 181.2 nm and + 37.2 my, respectively. Initially, toxicity profile was tested in zebrafish embryo at 96 h post fertilization (hpf) stage using medium molecular weight chitosan (MMW-C) and CNPs. At 5 mu g/mL, the hatching rate was almost similar in both treatments, however, the survival rate was lower in MMW-C compared to GNPs exposure, suggesting that toxicity effect of CNPs in hatched larvae was minimal at 5 mu g/mL compared to MMW-C. Quantitative real time PCR results showed that in CNPs exposed larvae at 5 days post fertilization (5 dpf) stage, immune related (il-1 beta, tnf-alpha, it-6, il-10, cxcl-18b, cc134a.4, cxcl-8a, lyz-c, def beta l-1, irf-1a, irf-3, MxA) and stress response (hsp-70) genes were induced. In contrast, basal or down regulated expression of antioxidant genes (gstp-1, cat, sod-1, prdx-4, txndr-1) were observed. Moreover, zebrafish larvae (at 5 dpf stage) exposed to CNPs (5 mu g/mL) showed higher survival rate at 72 h post infection stage against pathogenic Aeromonas hydrophila challenge compared to controls. These results suggest that although CNPs can have toxic effects to the larvae at higher doses, CNPs exposure at 5 mu g/mL could enhance the immune responses and develop the disease resistance against A. hydrophila, which could be attributed to its strong immune modulatory properties.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/939
DOI
10.1016/j.fsi.2018.03.010
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.76, pp.240 - 246, 2018
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
EPINEPHELUS-BRUNEUS; DRUG-DELIVERY; DANIO-RERIO; IN-VITRO; TOXICITY; VACCINE; SYSTEM; CHITIN; MODEL; FISH
Keywords
Chitosan nanoparticles; Zebrafish larvae; Immune response; Aeromonas hydrophila
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Recombinant protein,Marine microorganisms,Functional proteins,재조합단백질,해양미생물,기능성단백질

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse