Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Nikapitiya, Chamilani | - |
dc.contributor.author | Dananjaya, S. H. S. | - |
dc.contributor.author | De Silva, B. C. J. | - |
dc.contributor.author | Heo, Gang-Joon | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | De Zoysa, Mahanama | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-16T08:55:21Z | - |
dc.date.available | 2020-04-16T08:55:21Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/939 | - |
dc.description.abstract | Chitosan nanoparticles (CNPs) were synthesized by ionic gelation method and its immunomodulatory properties were investigated in zebrafish larvae. Average particle size and zeta potential were 181.2 nm and + 37.2 my, respectively. Initially, toxicity profile was tested in zebrafish embryo at 96 h post fertilization (hpf) stage using medium molecular weight chitosan (MMW-C) and CNPs. At 5 mu g/mL, the hatching rate was almost similar in both treatments, however, the survival rate was lower in MMW-C compared to GNPs exposure, suggesting that toxicity effect of CNPs in hatched larvae was minimal at 5 mu g/mL compared to MMW-C. Quantitative real time PCR results showed that in CNPs exposed larvae at 5 days post fertilization (5 dpf) stage, immune related (il-1 beta, tnf-alpha, it-6, il-10, cxcl-18b, cc134a.4, cxcl-8a, lyz-c, def beta l-1, irf-1a, irf-3, MxA) and stress response (hsp-70) genes were induced. In contrast, basal or down regulated expression of antioxidant genes (gstp-1, cat, sod-1, prdx-4, txndr-1) were observed. Moreover, zebrafish larvae (at 5 dpf stage) exposed to CNPs (5 mu g/mL) showed higher survival rate at 72 h post infection stage against pathogenic Aeromonas hydrophila challenge compared to controls. These results suggest that although CNPs can have toxic effects to the larvae at higher doses, CNPs exposure at 5 mu g/mL could enhance the immune responses and develop the disease resistance against A. hydrophila, which could be attributed to its strong immune modulatory properties. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.subject | EPINEPHELUS-BRUNEUS | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | DANIO-RERIO | - |
dc.subject | IN-VITRO | - |
dc.subject | TOXICITY | - |
dc.subject | VACCINE | - |
dc.subject | SYSTEM | - |
dc.subject | CHITIN | - |
dc.subject | MODEL | - |
dc.subject | FISH | - |
dc.title | Chitosan nanoparticles: A positive immune response modulator as display in zebrafish larvae against Aeromonas hydrophila infection | - |
dc.type | Article | - |
dc.citation.endPage | 246 | - |
dc.citation.startPage | 240 | - |
dc.citation.title | FISH & SHELLFISH IMMUNOLOGY | - |
dc.citation.volume | 76 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.identifier.bibliographicCitation | FISH & SHELLFISH IMMUNOLOGY, v.76, pp.240 - 246 | - |
dc.identifier.doi | 10.1016/j.fsi.2018.03.010 | - |
dc.identifier.scopusid | 2-s2.0-85043383910 | - |
dc.identifier.wosid | 000430888400029 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | EPINEPHELUS-BRUNEUS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | DANIO-RERIO | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | VACCINE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CHITIN | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | FISH | - |
dc.subject.keywordAuthor | Chitosan nanoparticles | - |
dc.subject.keywordAuthor | Zebrafish larvae | - |
dc.subject.keywordAuthor | Immune response | - |
dc.subject.keywordAuthor | Aeromonas hydrophila | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |