Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae
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Title
- Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae
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Author(s)
- Oh, Baek-Rock; Lee, Sung-Mok; Heo, Sun-Yeon; Seo, Jeong-Woo; Kim, Chul Ho
- KIOST Author(s)
- Lee, Sung Mok(이성목)
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Alternative Author(s)
- 이성목
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Publication Year
- 2018-06-15
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Abstract
- Background: 1,3-Propanediol (1,3-PDO) is important building blocks for the bio-based chemical industry, Klebsiella pneumoniae can be an attractive candidate for their production. However, 1,3-PDO production is high but productivity is generally low by K. pneumoniae. In this study, repeated fed-batch cultivation by a lactate and 2,3-butanediol (2,3-BDO) deficient mutant of K. pneumoniae were investigated for efficient 1,3-PDO production from industrial by-products such as crude glycerol. Results: First, optimal conditions for repeated fed-batch fermentation of a Delta ldhA mutant defective for lactate formation due to deletion of the lactate dehydrogenase gene (ldhA) were determined. Maximal 1,3-PDO production level and productivity obtained by repeated fed-batch fermentation under optimized conditions were 81.1 g/L and 3.38 g/L/h, respectively, and these values were successfully maintained for five cycles of fermentation without any loss of fermentation capacity. This results were much higher than that of the normal fed-batch fermentation. The levels of 2,3-BDO, which is a major by-product, reaching up to similar to 50% of the level of 1,3-PDO, were reduced using a mutant strain [Delta(ldhA als)] containing an additional mutation in the biosynthetic pathway of 2,3-BDO (deletion of the acetolactate synthase gene). The levels of 2,3-BDO were reduced to about 20% of 1,3-PDO levels by repeated fed-batch fermentation of Delta(ldhA als), although maximal 1,3-PDO production and productivity also decreased owing to a defect in the growth of the 2,3-BDO-defective mutant strain. Conclusion: This repeated fed-batch fermentation may be useful for reducing the cost of 1,3-PDO production and may be promising industrialization prospect for the 1,3-PDO production.
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ISSN
- 1475-2859
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/875
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DOI
- 10.1186/s12934-018-0921-z
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Bibliographic Citation
- MICROBIAL CELL FACTORIES, v.17, 2018
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Publisher
- BIOMED CENTRAL LTD
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Subject
- CULTURE-CONDITIONS; ESCHERICHIA-COLI; INACTIVATION; OPTIMIZATION
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Keywords
- Klebsiella pneumoniae; Crude glycerol; 1,3-Propanediol; Repeated fed-batch fermentation; Mutant
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Type
- Article
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Language
- English
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Document Type
- Article
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