Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Oh, Baek-Rock | - |
dc.contributor.author | Lee, Sung-Mok | - |
dc.contributor.author | Heo, Sun-Yeon | - |
dc.contributor.author | Seo, Jeong-Woo | - |
dc.contributor.author | Kim, Chul Ho | - |
dc.date.accessioned | 2020-04-16T08:55:03Z | - |
dc.date.available | 2020-04-16T08:55:03Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2018-06-15 | - |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/875 | - |
dc.description.abstract | Background: 1,3-Propanediol (1,3-PDO) is important building blocks for the bio-based chemical industry, Klebsiella pneumoniae can be an attractive candidate for their production. However, 1,3-PDO production is high but productivity is generally low by K. pneumoniae. In this study, repeated fed-batch cultivation by a lactate and 2,3-butanediol (2,3-BDO) deficient mutant of K. pneumoniae were investigated for efficient 1,3-PDO production from industrial by-products such as crude glycerol. Results: First, optimal conditions for repeated fed-batch fermentation of a Delta ldhA mutant defective for lactate formation due to deletion of the lactate dehydrogenase gene (ldhA) were determined. Maximal 1,3-PDO production level and productivity obtained by repeated fed-batch fermentation under optimized conditions were 81.1 g/L and 3.38 g/L/h, respectively, and these values were successfully maintained for five cycles of fermentation without any loss of fermentation capacity. This results were much higher than that of the normal fed-batch fermentation. The levels of 2,3-BDO, which is a major by-product, reaching up to similar to 50% of the level of 1,3-PDO, were reduced using a mutant strain [Delta(ldhA als)] containing an additional mutation in the biosynthetic pathway of 2,3-BDO (deletion of the acetolactate synthase gene). The levels of 2,3-BDO were reduced to about 20% of 1,3-PDO levels by repeated fed-batch fermentation of Delta(ldhA als), although maximal 1,3-PDO production and productivity also decreased owing to a defect in the growth of the 2,3-BDO-defective mutant strain. Conclusion: This repeated fed-batch fermentation may be useful for reducing the cost of 1,3-PDO production and may be promising industrialization prospect for the 1,3-PDO production. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject | CULTURE-CONDITIONS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | INACTIVATION | - |
dc.subject | OPTIMIZATION | - |
dc.title | Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae | - |
dc.type | Article | - |
dc.citation.title | MICROBIAL CELL FACTORIES | - |
dc.citation.volume | 17 | - |
dc.contributor.alternativeName | 이성목 | - |
dc.identifier.bibliographicCitation | MICROBIAL CELL FACTORIES, v.17 | - |
dc.identifier.doi | 10.1186/s12934-018-0921-z | - |
dc.identifier.scopusid | 2-s2.0-85048683120 | - |
dc.identifier.wosid | 000435420600001 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CULTURE-CONDITIONS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | Crude glycerol | - |
dc.subject.keywordAuthor | 1,3-Propanediol | - |
dc.subject.keywordAuthor | Repeated fed-batch fermentation | - |
dc.subject.keywordAuthor | Mutant | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |