Specific detection of an oil-degrading bacterium, Corynebacterium sp IC10, in sand microcosms by PCR using species-specific primers based on 16S rRNA gene sequences SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 6 time in Scopus
Title
Specific detection of an oil-degrading bacterium, Corynebacterium sp IC10, in sand microcosms by PCR using species-specific primers based on 16S rRNA gene sequences
Author(s)
Lee, JH; Jung, SY; Kim, SJ
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2001
Abstract
A species-specific PCR technique to detect an oil-degrading bacterium, Corynebacterium sp. IC10, released into sand microcosms is described. PCR primers, specific to strain IC10, were designed based on 16S rRNA gene sequences and tested against both closely and distantly related bacterial strains using four primer combinations involving two forward and two reverse primers. Two sets of them were specific to the strain IC10 and Corynebacterium variabilis and one set was selected for further analysis. The PCR amplification was able to detect 1 pg template DNA of strain IC10 and 1.2x10(4) c.f.u. of IC10 ml wet sand(-1) in the presence of 3x10(8) Escherichia coli cells. In non-sterile sand microcosms seeded with the strain IC10, the sensitivity of detection decreased to 9.6x10(5) c.f.u. ml wet sand(-1). The detection sensitivity thus depends on the complexity of background heterogeneous DNA of environmental samples. The assay is suitable for detection of Corynebacterium sp. IC10 in laboratory microcosms, however, cross reaction with non-oil degrading coryneforms may prohibit its use in uncharacterized systems.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5929
DOI
10.1023/A:1012440232320
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.23, no.21, pp.1741 - 1748, 2001
Publisher
KLUWER ACADEMIC PUBL
Subject
MICROBIAL-CELLS; IDENTIFICATION; PHENANTHRENE; DNA
Keywords
16S rDNA; Corynebacterium sp.; oil-degradation; species specific-PCR
Type
Article
Language
English
Document Type
Article
Publisher
KLUWER ACADEMIC PUBL
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse