남극에서 분리한 저온성 세균 유래 단백질 분해효소

Abstract

저온에서 최적 생육을 보이는 저온성 균주를 남극해양에서 분리하여 생화학적 특성 및 165 rRNA 염기서열로부터 Shewanella sp.에 속하는 균주로 동정하고 Shewanella sp. L93으로명명하였다. 본 균주에서 생산되는 저온성 세포외 단백질 분해 효소(extracellular protease)를 ammonium sulfate precipitation, High-Q column chromatography, 일차 gel permeation chromatography, BioScale Q2 ion exchange chromatography 및 gel permeation chromatography를 통하여 purification fold 19.3, yield 0.7 %로 정제하였고 그 특성을 조사하였다.

A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.