Cephamycin C production is regulated by relA and rsh genes in Streptomyces clavuligerus ATCC27064 SCIE SCOPUS

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Title
Cephamycin C production is regulated by relA and rsh genes in Streptomyces clavuligerus ATCC27064
Author(s)
Jin, W; Kim, HK; Kim, JY; Kang, SG; Lee, SH; Lee, KJ
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2004-10-19
Abstract
The effects of growth rate and nutrient uptake rate on the production of cephamycin C were determined in the parental strain, DeltarelA mutant, and Deltarsh null mutant of S. clavuligerus. Production of cephamycin C was inversely related to mycelium growth and the phosphate feed rate was more critical for the production of cephmycin C. On the contrary, the production of cephamycin C was completely abolished in the DeltarelA mutant, but not in Deltarsh mutant. The changes in the cephamycin C production by disruption of the relA and rsh genes are presumably associated with the consequent ability of the mutants to accumulate (p)ppGpp under nutrient starvation. Therefore, it is concluded that the stringent response of S. clavuligerus to starvation for nutrients is governed mainly by RelA rather than Rsh and that the response is more apparently regulated by the limitation of phosphate. (C) 2004 Elsevier B.V. All rights reserved.
ISSN
0168-1656
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5202
DOI
10.1016/j.jbiotec.2004.06.010
Bibliographic Citation
JOURNAL OF BIOTECHNOLOGY, v.114, no.1-2, pp.81 - 87, 2004
Publisher
ELSEVIER SCIENCE BV
Subject
STREPTOMYCES-COELICOLOR A3(2); STRINGENT RESPONSE; PHYSIOLOGICAL DIFFERENTIATION; ANTIBIOTIC PRODUCTION; FUNCTIONAL-ANALYSIS; ESCHERICHIA-COLI; CLAVULIGERUS; PPGPP; INITIATION; EXTRACTS
Keywords
cephamycin C; relA and rsh gene; (p)ppGpp; nutrient starvation
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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