Optimization of shark (Squatina oculata) cartilage hydrolysis for the preparation of chondroitin sulfate
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Title
- Optimization of shark (Squatina oculata) cartilage hydrolysis for the preparation of chondroitin sulfate
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Author(s)
- Jo, JH; Do, JR; Kim, YM; Kim, DS; Lee, TK; Kim, SB; Cho, SM; Kang, SN; Park, DC
- KIOST Author(s)
- Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 이택견
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Publication Year
- 2005-10
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Abstract
- Enzymatic hydrolysis of shark (Squatina oculata) cartilage (SC) was optimized by response surface methodology (RSM) for chondroitin sulfate (CS) preparation. Among I I commerical proteases, Maxazyme NNP showed highest productivity (CS yield per enzyme cost) of CS. Optimal hydrolysis conditions determined by RSM were 1.63% and 2.87 hr For enzyme concentration and hydrolysis time (r(2) = 0.9527, p < 0.01), respectively and highest yield of hydrolysate under the conditions was 42.3%. The yield (43.1 +/- 2.1%) and CS content (24.8 +/- 0.1%) of hydrolysate at optimal condition verified statistical optimization of SC enzymatic hydrolysis was valid.
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ISSN
- 1226-7708
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/5020
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Bibliographic Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.14, no.5, pp.651 - 655, 2005
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Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
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Keywords
- chondroitin sulfate; shark cartilage; hydrolysis optimization; RSM
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Type
- Article
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Language
- English
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Document Type
- Article
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