Monitoring of microorganisms added into oil-contaminated microenvironments by terminal-restriction fragment length polymorphism analysis SCIE SCOPUS KCI

Cited 8 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Monitoring of microorganisms added into oil-contaminated microenvironments by terminal-restriction fragment length polymorphism analysis
Author(s)
Jung, SY; Lee, JH; Chai, YG; Kim, SJ
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2005-12
Abstract
Term inal -restriction fragment length polymorphism (T-RFLP) analysis was used to monitor inoculated oil-degrading microorganisms during bioremedial treatability tests. A pair of universal primers, fluorescently labeled 521F and 1392R, was employed to amplify small subunit rDNA in order to simultaneously detect two bacterial strains, Corynebacterium sp. IC10 and Sphingomonas sp. KH3-2, and a yeast strain, Yarrowia lipolytica 180. Digestion of the 5 '-end fluorescence-labeled PCR products with HhaI produced specific terminal-restriction fragments (T-RFs) of 185 and 442 bases, corresponding to Corynebacterium sp. IC 10 and Y lipolytica 180, respectively. The enzyme NruI produced a specific T-RF of 338 bases for Sphingomonas sp. KH3-2. The detection limit for oil-degrading microorganisms that were inoculated into natural environments was determined to be 0.01% of the total microbial count, regardless of the background environment. When three oil-degrading microorganisms were released into oil-contaminated sand microenvironments, strains IC10 and 180 survived for 35 days after inoculation, whereas strain KH3-2 was detected at 8 days, but not at 3 5 days. This result implies that T-RFLP could be a useful tool for monitoring the survival and relative abundance of specific microbial strains inoculated into contaminated environments.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5001
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.15, no.6, pp.1170 - 1177, 2005
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
16S RIBOSOMAL-RNA; BACTERIAL COMMUNITY STRUCTURE; POLYMERASE CHAIN-REACTION; SPECIES-SPECIFIC PRIMERS; YARROWIA-LIPOLYTICA 180; MICROBIAL DIVERSITY; ACTIVATED-SLUDGE; T-RFLP; DEGRADING YEAST; COPY NUMBER
Keywords
oil-degrading microorganisms; specific detection; terminal-restriction fragment length polymorphism (T-RFLP)
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
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