Enantioselective epoxide hydrolase activity of a newly isolated microorganism, Sphingomonas echinoides EH-983, from seawater SCIE SCOPUS

Cited 18 time in WEB OF SCIENCE Cited 19 time in Scopus
Title
Enantioselective epoxide hydrolase activity of a newly isolated microorganism, Sphingomonas echinoides EH-983, from seawater
Author(s)
Kim, Hee Sook; Lee, Ok Kyung; Lee, Soo Jung; Hwang, Seungha; Kim, Sang Jin; Yang, Sung-Hyun; Park, Sunghoon; Lee, Eun Yeol
Publication Year
2006-08-04
Abstract
A marine microorganism, Sphingomonas echinoides EH-983, which possesses epoxide hydrolase (EH) activity was isolated from seawater and characterized. The EH of S. echinoides EH-983 preferentially metabolized (R)-enantiomer when the racemic styrene oxides were supplied as substrates. The optimal pH and temperature for the enantioselective hydrolysis by whole-cells of S. echinoides EH-983 were 7.0 and 20 degrees C, respectively. When kinetic resolution was conducted with a racemic mixture of styrene oxides at an initial concentration of 40 mM, enantiopure (S)-styrene oxide was obtained in 180 min with a yield of 21.3%. To our best knowledge, S. echinoides EH-983 is the first marine microorganism that is reported to have EH activity. (c) 2006 Elsevier B.V. All rights reserved.
ISSN
1381-1177
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4855
DOI
10.1016/j.molcatb.2006.05.009
Bibliographic Citation
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.41, no.3-4, pp.130 - 135, 2006
Publisher
ELSEVIER SCIENCE BV
Subject
RHODOTORULA-GLUTINIS; KINETIC RESOLUTION; SP-NOV.; HYDROLYSIS; OXIDE; ASSAY
Keywords
enantioselective hydrolysis; epoxide hydrolase; Sphingomonas echinoides EH-983; marine microorganism
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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