Enantioselective epoxide hydrolase activity of a newly isolated microorganism, Sphingomonas echinoides EH-983, from seawater
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Title
- Enantioselective epoxide hydrolase activity of a newly isolated microorganism, Sphingomonas echinoides EH-983, from seawater
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Author(s)
- Kim, Hee Sook; Lee, Ok Kyung; Lee, Soo Jung; Hwang, Seungha; Kim, Sang Jin; Yang, Sung-Hyun; Park, Sunghoon; Lee, Eun Yeol
- KIOST Author(s)
- Yang, Sung Hyun(양성현)
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Alternative Author(s)
- 김상진; 양성현
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Publication Year
- 2006-08-04
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Abstract
- A marine microorganism, Sphingomonas echinoides EH-983, which possesses epoxide hydrolase (EH) activity was isolated from seawater and characterized. The EH of S. echinoides EH-983 preferentially metabolized (R)-enantiomer when the racemic styrene oxides were supplied as substrates. The optimal pH and temperature for the enantioselective hydrolysis by whole-cells of S. echinoides EH-983 were 7.0 and 20 degrees C, respectively. When kinetic resolution was conducted with a racemic mixture of styrene oxides at an initial concentration of 40 mM, enantiopure (S)-styrene oxide was obtained in 180 min with a yield of 21.3%. To our best knowledge, S. echinoides EH-983 is the first marine microorganism that is reported to have EH activity. (c) 2006 Elsevier B.V. All rights reserved.
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ISSN
- 1381-1177
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4855
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DOI
- 10.1016/j.molcatb.2006.05.009
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Bibliographic Citation
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.41, no.3-4, pp.130 - 135, 2006
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Publisher
- ELSEVIER SCIENCE BV
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Subject
- RHODOTORULA-GLUTINIS; KINETIC RESOLUTION; SP-NOV.; HYDROLYSIS; OXIDE; ASSAY
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Keywords
- enantioselective hydrolysis; epoxide hydrolase; Sphingomonas echinoides EH-983; marine microorganism
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Type
- Article
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Language
- English
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Document Type
- Article
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