Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus
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Title
- Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus
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Author(s)
- Lee, Soo Jung; Kim, Hee Sook; Kim, Sang Jin; Park, Sunghoon; Kim, Beum Jun; Shuler, Michael L.; Lee, Eun Yeol
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Alternative Author(s)
- 김상진
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Publication Year
- 2007-02
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Abstract
- The cDNA of a marine fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was cloned by rapid amplification of cDNA ends (RACE) techniques. The homology model for the mEH of M. cephalus showed a characteristic structure of alpha/beta-hydrolase-fold main domain with a lid domain over the active site. The characteristic catalytic triad, consisting of Asp(238), His(444), and Glu(417), was highly conserved. The cloned mEH gene was expressed in Escherichia coli and the recombinant mEH exhibited (R)-preferred hydrolysis activity toward racemic styrene oxide. We obtained enantiopure (S)-styrene oxide with a high enantiopurity of more than 99% enantiomeric excess and yield of 15.4% by batch kinetic resolution of 20 mM racemic styrene oxide.
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ISSN
- 0141-5492
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4739
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DOI
- 10.1007/s10529-006-9222-4
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Bibliographic Citation
- BIOTECHNOLOGY LETTERS, v.29, no.2, pp.237 - 246, 2007
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Publisher
- SPRINGER
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Subject
- RACEMIC STYRENE OXIDE; KINETIC RESOLUTION; RHODOSPORIDIUM; RHODOTORULA; METABOLISM; ROLES
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Keywords
- cDNA cloning; enantiopure epoxides; marine fish epoxide hydrolase; Mugil cephalus; RACE
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Type
- Article
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Language
- English
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Document Type
- Article
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