Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 19 time in Scopus
Title
Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus
Author(s)
Lee, Soo Jung; Kim, Hee Sook; Kim, Sang Jin; Park, Sunghoon; Kim, Beum Jun; Shuler, Michael L.; Lee, Eun Yeol
Publication Year
2007-02
Abstract
The cDNA of a marine fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was cloned by rapid amplification of cDNA ends (RACE) techniques. The homology model for the mEH of M. cephalus showed a characteristic structure of alpha/beta-hydrolase-fold main domain with a lid domain over the active site. The characteristic catalytic triad, consisting of Asp(238), His(444), and Glu(417), was highly conserved. The cloned mEH gene was expressed in Escherichia coli and the recombinant mEH exhibited (R)-preferred hydrolysis activity toward racemic styrene oxide. We obtained enantiopure (S)-styrene oxide with a high enantiopurity of more than 99% enantiomeric excess and yield of 15.4% by batch kinetic resolution of 20 mM racemic styrene oxide.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4739
DOI
10.1007/s10529-006-9222-4
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.29, no.2, pp.237 - 246, 2007
Publisher
SPRINGER
Subject
RACEMIC STYRENE OXIDE; KINETIC RESOLUTION; RHODOSPORIDIUM; RHODOTORULA; METABOLISM; ROLES
Keywords
cDNA cloning; enantiopure epoxides; marine fish epoxide hydrolase; Mugil cephalus; RACE
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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