Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180 SCIE SCOPUS

Cited 46 time in WEB OF SCIENCE Cited 51 time in Scopus
Title
Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180
Author(s)
Kim, Jun-Tae; Kang, Sung Gyun; Woo, Jung-Hee; Lee, Jung-Hyun; Jeong, Byeong Chul; Kim, Sang-Jin
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2007-03
Abstract
To develop an enantioselective lipase/esterase hydrolyzing racemic ofloxacin ester to levofloxacin, samples were collected from a variety of marine environments such as cold sea, hydrothermal vent area, sediment, tidal flat area, arctic sea, marine organisms, and so on. Microorganisms were isolated by plating on an enrichment medium with simultaneous detection of lipolytic activities and screened for the hydrolysis of ofloxacin ester. Three candidates among isolates were selected, and one of them, identified as Yarrowia lipolytica CL180, hydrolyzed preferentially S-enantiomer of racemic ofloxacin ester. The lipase/esterase gene (yli180) was cloned by screening a genomic library. The sequence analysis revealed an open reading frame consisting of 1,431 bp that encoded a protein of 476 amino acids with a molecular mass of 53 kDa. The yli180 gene was expressed in Escherichia coli and purified to homogeneity. The optimum activity of the recombinant protein (rYli180) occurred at pH 7.5 and 35 degrees C, respectively. rYli180 preferentially hydrolyzed p-nitrophenyl esters of fatty acids with short chain lengths of <= 10 carbon atoms. This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester.
ISSN
0175-7598
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4729
DOI
10.1007/s00253-006-0727-5
Bibliographic Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.74, no.4, pp.820 - 828, 2007
Publisher
SPRINGER
Subject
OIL-DEGRADING YEAST; CHIRAL DRUGS; LIPASES; OFLOXACIN; ENZYMES; IDENTIFICATION; BIOTECHNOLOGY; EVOLUTION; DR-3355
Keywords
cloning; enantioselective lipase; marine microorganism; characterization; ofloxacin
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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