Screening and characterization of a novel fibrinolytic metalloprotease from a metagenomic library SCIE SCOPUS

Cited 49 time in WEB OF SCIENCE Cited 59 time in Scopus
Title
Screening and characterization of a novel fibrinolytic metalloprotease from a metagenomic library
Author(s)
Lee, Dong-Geun; Jeon, Jeong Ho; Jang, Min Kyung; Kim, Nam Young; Lee, Jong Hyun; Lee, Jung-Hyun; Kim, Sang-Jin; Kim, Gun-Do; Lee, Sang-Hyeon
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2007-03
Abstract
A metagenomic library was constructed using total genomic DNA extracted from the mud in the west coast of Korea and was used together with a fosmid vector, pCC1FOS in order to uncover novel gene sources. One clone from approximately 30,000 recombinant Escherichia coli clones was identified that showed proteolytic activity. The gene for the proteolytic enzyme was subcloned into pUC19 and sequenced, and a database search for homologies revealed it to be a zinc-dependent metalloprotease. The cloned gene included the intact coding gene for a novel metalloproteinase and its own promoter. It comprised an open reading frame of 1,080 base pairs, which encodes a protein of 39,490 Da consisting of 359 amino acid residues. A His-Glu-X-X-His sequence, which is a conserved sequence in the active site of zinc-dependent metalloproteases, was found in the deduced amino acid sequence of the gene, suggesting that the enzyme is a zinc-dependent metalloprotease. The purified enzyme showed optimal activity at 50 degrees C for 1 h and pH 7.0. The enzyme activity was inhibited by metal-chelating reagents, such as EDTA, EGTA and 1,10-phenanthroline. The enzyme hydrolyzed azocasein as well as fibrin. Thus, the enzyme could be useful as a therapeutic agent to treat thrombosis.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4726
DOI
10.1007/s10529-006-9263-8
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.29, no.3, pp.465 - 472, 2007
Publisher
SPRINGER
Subject
ESCHERICHIA-COLI; FERMENTED FOOD; SNAKE-VENOM; ENZYME; IDENTIFICATION; PURIFICATION; EXPRESSION; PROTEIN; RNA
Keywords
azocasein; fibrinolytic; metagenome; metalloprotease
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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